Services and Equipment

Services:

• Project management and experimental design
• Patient consent and sample collection logistics
• Aerobic and anaerobic culture
• Microbial identification and analysis
• Phenotypic analyses include short-chain fatty acid (SCFA) profiles in primary samples or cultured isolates
• Preparation of materials for 16S rRNA microbiome analyses
• Bioinformatic and computational analyses of metagenomic datasets
• Time series analyses of complex microbial ecosystems leveraging novel Bayesian models developed by core faculty

Equipment and Instrumentation:

• Laboratory Information Management System (LIMS) for patient registration, sample submission and viewing of datasets
• The core maintains a complement of 22 isolators of various configurations for breeding and experiments under germfree, BL1 or BL2 conditions
• 100-cage containment system for short-term GF and long-term defined colonization studies
• Anaerobic hood and automated microbiological identification systems
• Automated DNA extraction and library preparation equipment
• qPCR and Illumina NGS platforms
• HIPAA-compliant computational cluster
• Gnotobiotic (germfree) mice
• Gnotobiotic and defined colonization experiments in gnotobiotic isolators or high-throughput cage containment systems
• Assistance with mouse necropsy and tissue preparation for histopathology

Fees: To receive a quote for utilization of services please contact the core's Project Manager or fill in the Core's Request Form: Click here to download Form

Project Manager:  Vladimir Yeliseyev

Telephone Number:  (617) 525-7180

Email: VYELISEYEV@PARTNERS.ORG