Core Services

Overview of HDDC Cores and Services

Use the links for detailed descriptions, locations and contact information.

Biomedical CORE B: Microscopy and Histopathology

  • Paraffin histology
  • Electron microscopy
  • Confocal microscopy (conventional or spinning disc)
  • Widefield fluorescence microscopy
  • Immunostaining (includes frozen sectioning)
  • Photodocumentation, image analysis and processing
  • More Information...

Biomedical CORE C: Epithelial Cell and Mucosal Immunology

  • Polarized epithelial cell culture
  • Genetic transformations of epithelial and other cells
  • Intestinal organoid conditioned media
  • FACs 12-Color analysis of cultured cells or tissue/organoid-derived cells
  • Transepithelial electrophysiology of live cells, cell monolayers, and tissues
  • Gastrointestinal organoid derivation and culture
  • More Information...

Biomedical CORE D: Gnotobiotic Mice, Microbiology and Metagenomics

  • Nucleic acid extraction/amplification
  • Microbiome and microbial genome sequencing
  • Microbiology
  • Gnotobiotic mice, hosing, defined and complex associations
  • Computation and data analysis
  • Metabolic cages and monitoring, under conventional and GF states
  • More Information...

Clinical Translational Research Program

  • Design of clinical and preclinical studies
  • Statistical analysis
  • Data interpretation and presentation
  • More Information...

Bio-Repository and Data Registry for Human Material

  • Fresh and stored human gastrointestinal tissues, stool, and blood
  • Sample-associated clinical metadata
  • More Information...

Who can use the HDDC Cores and services?

 

Please remember to acknowledge the Harvard Digestive Disease Center and NIH grant P30DK034854 in all publications and presentations emerging from HDDC core services, resources, technology, and expertise.